This report describes the production of ERM-AD484ka, b and c, which is a set of materials certified for the copy number concentration of a poultry plasmid DNA, produced within the scope of ISO 17034 accreditation. A DNA fragment specific for the identification of a poultry target was cloned into a vector to construct the poultry plasmid. The nucleic acid sequence of the entire poultry plasmid was determined by dye terminator cycle sequencing. The plasmid was eluted in buffer and gravimetrically diluted to three different concentration levels. The copy number concentration of the three concentration levels was certified by digital PCR methods. The CRM is available as a set of three polypropylene vials containing at least 1 mL of plasmid solution. The material was characterised by an interlaboratory comparison of laboratories of demonstrated competence and adhering to ISO/IEC 17025:2017. Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation. The material is intended for the determination of a cut-off value to discriminate positive samples (containing the poultry target sequence) from negative samples by quantitative PCR as defined in the standard operating procedure of the European Union Reference Laboratory for the detection of Animal Proteins in feedingstuffs according to Commission Regulation (EU) No 51/2013.

MEEUS Nele;  MAZOUA Stephane;  TRAPMANN Stefanie; 

2022-01-18

Publications Office of the European Union

JRC127664

978-92-76-45338-3 (online),   

1831-9424 (online),   

EUR 30928 EN,    OP KJ-NA-30928-EN-N (online),   

https://publications.jrc.ec.europa.eu/repository/handle/JRC127664,   

10.2760/8921 (online),