DT-Diaphorase Affects the Mutagenic Activity of Pyrene 1.6-Quinone in Chinese Hamster Epithelial Liver (CHEL) Cells

The mutagenic potential of pyrene 1,6-quinone has been studied in a wide range of in vitro genetic assays using mammalian cell lines. Pyrene 1,6-quinone induced gene mutations and micronuclei in V79 cells whereas in CHEL cells, an epithelial liver cell line which retained activities of various xenobiotic-metabolizing enzymes, a non-specific pattern of structural and numerical chromosomal aberrations were observed. In this study we have evaluated the mutagenic activity of pyrene 1,6-quinone on V79 and CHEL cells in presence and absence of the dicoumarol, a potent inhibitor of DT-diaphorase. In V79 cells dicoumarol (100um) did not affect the mutagenic responce, whereas in CHEL cells the mutation frequency significantly increased to suggest the possible role of DT-diaphorase, expressed in the liver cells at high levels, in the detoxification of pyrene 1,6-quinone to redox-stable hydroquinone.

ROSSINI Paola;  TURCHI Gino; 

1996-04-16

JRC12830

https://publications.jrc.ec.europa.eu/repository/handle/JRC12830,