The certification of different mass fractions of MIR162 in maize seed powder
ERM®-BF446a, ERM®-BF446b, ERM®-BF446c, ERM® BF446d and ERM®-BF446e
This report describes the production of ERM®-BF446, maize seed powder materials certified for the mass fraction of MIR162 (unique identifier SYN-IR162-4). These materials were produced and certified in accordance with ISO 17034:2016 and ISO Guide 35:2017. ERM- BF446 was produced within the scope of ISO 17034:2016 accreditation.
Genetically modified (GM) maize seeds of the MIR162 event and seeds from a non-GM maize variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize seed powder were prepared gravimetrically.
The certified reference materials (CRMs) are available in glass vials containing at least 1 g of dried maize seed powder, which were sealed under an atmosphere of argon.
Between-unit homogeneity was quantified and stability during dispatch and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg.
The certified value was obtained from the gravimetric preparations, taking into account the genetic purity of the base materials with respect to the MIR162 maize and their water mass fractions. The certified values were confirmed by event-specific real-time polymerase chain reaction (PCR) (measurements performed adhering ISO/IEC 17025:2017).
Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation.
KORTEKAAS Anna Maria;
SEGHERS John;
GARCIA RUIZ Silvia;
CONTRERAS LOPEZ Maria Concepcion;
CORBISIER Philippe;
TRAPMANN Stefanie;
2022-06-27
Publications Office of the European Union
JRC129454
978-92-76-52453-3 (online),
1831-9424 (online),
EUR 31076 EN,
OP KJ-NA-31076-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC129454,
10.2760/201469 (online),
Additional supporting files
| File name | Description | File type | |
|---|
