Certification of different mass fractions of DP23211 in maize seed powder: ERM®-BF445a and ERM®-BF445b
ERM®-BF445a and ERM®-BF445b are matrix materials certified for the mass fraction of DP23211 maize (unique identifier DP-Ø23211-2), produced within the scope of ISO 17034 accreditation and ISO Guide 35:2017.
Genetically modified (GM) maize seeds of the DP23211 event and seeds from a non-GM maize variety were milled, sieved and dried to obtain GM and non-GM seed powders. The CRMs are available in glass vials containing at least 1 g of dried maize powder, which were sealed under an atmosphere of argon.
The purity of the non-GM and GM maize seeds and the respective maize powders was quantified. The stability during transport and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg.
The certified value was obtained, taking into account the genetic purity of the base materials with respect to the DP23211 maize. The certified values were confirmed by event-specific real-time polymerase chain reaction (PCR) (measurements were within the scope of accreditation to ISO/IEC 17025:2017)
Technically invalid results were removed but no outlier was eliminated unless a technical reason for the deviation was found.
Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 [2] and include uncertainties related to the genetic purity assessment.
KORTEKAAS Anna Maria;
SEGHERS John;
GARCIA RUIZ Silvia;
CONTRERAS LOPEZ Maria Concepcion;
KHALED GIJON Mohamed;
CORBISIER Philippe;
TRAPMANN Stefanie;
2022-12-19
Publications Office of the European Union
JRC131609
978-92-76-59124-5 (online),
1831-9424 (online),
EUR 31317 EN,
OP KJ-NA-31-317-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC131609,
10.2760/066984 (online),
Additional supporting files
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