40 test items relevant to EU-NETVAL validation study of in vitro methods for the detection of thyroid hormone system disrupting chemicals
Nephelometry has been applied for systematic solubility determination of 40 chemicals selected as test items in a ring trial validation study of in vitro methods relevant to detection of thyroid hormone system disruption (THSD) potential of chemicals. Starting with 100mM DMSO stock solutions, for aliquot dilution 1000-fold to 100µM (abbreviated as C100) in 3 cell culture media, DMEM with/without serum and water, solubility/stability measurement was monitored during 72 hours incubation at 37°C. In cases of C100 insolubility (stock or medium) repeat measurements were made at corresponding dilutions C50, C25, and C10, and additionally at C5 (if C10 insoluble). Formazin reference standards (aqueous suspensions at 1, 5, 10 and 20 nephelometric turbidity units, NTU) were used for instrument calibration, producing sensitive, linear and reproducible response, and a basis for establishing thresholds (relative turbidity units, RTU) applicable to definition of test item solubility. Results obtained for the chemicals, either soluble, insoluble or at solubility limit, are presented in the report.
COLE Thomas;
FORTANER TORRENT Salvador;
LANGEZAAL Ingrid;
COECKE Sandra;
2023-03-15
Publications Office of the European Union
JRC132585
978-92-68-01052-5 (online),
1831-9424 (online),
EUR 31447 EN,
OP KJ-NA-31-447-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC132585,
10.2760/398195 (online),
