Multi‑omics characterization of NIST seafood reference materials and alternative matrix preparations

The National Institute of Standards and Technology (NIST) has prepared four seafood reference materials for use in food safety and authentication studies: wild-caught and aquacultured salmon (RM 8256 and RM 8257) and wild-caught and aquacultured shrimp (RM 8258 and RM 8259). These materials were characterized using genetic, metabolomic (1H-NMR and LC-HRMS/MS), lipidomic and proteomic methods to explore the use of these verified seafood products as matrix-matched quality control materials and as differential materials for identifying product source in untargeted omics studies. Additionally, differences between the materials could be detected based on source/species, where wild-caught and aquacultured seafood could be distinguished using untargeted metabolite, lipid and protein analyses. Further processing of the fresh frozen RMs by freeze-drying revealed that the freeze-dried seafoods could still be reliably discerned based on product source/species. These results demonstrate the potential usefulness of these reference materials as tools for omics instrument validation and measurement harmonization in seafood-related studies. Furthermore, their use as differential quality control materials, regardless of preparation method, may also provide a tool for laboratories to demonstrate proficiency at discriminating between products based on source/species in the field of product authentication.

ELLISOR Debra;  BAYLESS Amanda;  SCHOCK Tracey;  DAVIS Clay W;  KNOTT Trey;  SEGHERS John;  LEYS Hanne;  EMTEBORG Hakan; 

2024-01-16

SPRINGER HEIDELBERG

JRC133315

1618-2642 (online),   

https://doi.org/10.1007/s00216-023-04928-9,    https://publications.jrc.ec.europa.eu/repository/handle/JRC133315,   

10.1007/s00216-023-04928-9 (online),