Morphological and lipid metabolism alterations in macrophages exposed to model environmental nanoplastics traced by high-resolution synchrotron techniques

The release of nanoplastics in the environment is a major health concern for long-term exposed humans. Although their usage has certainly revolutionized several application fields, at nanometer size, NPs can easily interact at the cellular level, arising potential harmful effects. The amount of literature studies on the NPs presence in biological samples is increasing, but only a few offers a close study on the model environmental NPs-immune system interaction exploited by advanced microscopy techniques. The present study highlights a significant morphological and lipid metabolism alterations in human macrophages M1 exposed to PP-, PVC-NPs . The results are interpreted by advanced microscopy techniques, combined with standard laboratory tests and fluorescence microscopy. We report the accurate detection of CdSe-QDs NPs by following the Se (L line) X-ray emission peak at higher sub-cellular resolution, compared to the supportive light fluorescence microscopy

ZINGARO Federica;  GIANONCELLI Alessandra;  CECCONE Giacomo;  BIRARDA Giovanni;  CASSANO Domenico;  LA SPINA Rita;  AGOSTINIS Chiara;  BONANNI Valentina;  RICCI Giuseppe;  PASCOLO Lorella; 

2024-06-25

FRONTIERS MEDIA SA

JRC134283

1664-3224 (online),   

https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1247747,    https://publications.jrc.ec.europa.eu/repository/handle/JRC134283,   

10.3389/fimmu.2023.1247747 (online),