Polar pesticides in food of animal origin: interlaboratory validation to evaluate the fitness-for-purpose of an analytical method for official control

The present work reports on the design, execution and evaluation of results of an interlaboratory validation study aiming at verifying the fitness-for-purpose of a LC-MS/MS method for the detection of polar pesticides in food of animal origin within the frame of official control and monitoring programmes. To this scope, five participant laboratories, with relevant expertise, were recruited. After passing a pre-trial test, the participants were asked to analyze test samples of bovine fat, chicken eggs and cow milk, contaminated with 11 polar pesticides (AMPA, cyanuric acid, ethephon, glyphosate, fosetyl aluminium, 2-hydroxyethyphosphonic acid, maleic hydrazide, N-acetyl-glyphosate, N-acetyl glufosinate, 3-methylphosphinicopropionic acid and glufosinate ammonium). The method was based on acidified methanol/water extraction followed by dSPE clean up with C18 sorbent. For LC-MS/MS analysis isotopically labelled standards were used for all targeted analytes. With a couple of exceptions, average recoveries ranged from 85% to 110%, with repeatability (RSDr) ranging from 3% to 25%, and reproducibility (RSDR) from 4% to 26%. The assessment by different laboratories provided also insights on key factors influencing method performance characteristics and its implementation by new users.

LATTANZIO Veronica;  CIASCA Biancamaria;  VERDINI Emanuela;  VON HOLST Christoph;  PECORELLI Ivan; 

2023-12-05

TAYLOR & FRANCIS LTD

JRC134522

1944-0049 (online),   

https://www.tandfonline.com/doi/full/10.1080/19440049.2023.2255302,    https://publications.jrc.ec.europa.eu/repository/handle/JRC134522,   

10.1080/19440049.2023.2255302 (print),