Certification of different mass fractions of DP915635 in maize seed powder: ERM®-BF447a, ERM®-BF447b, ERM®-BF447c, ERM®-BF447d and ERM®-BF447e
Certified reference materials ERM®-BF447a, ERM®-BF447b, ERM®-BF447c, ERM®-BF447d and ERM®-BF447e
ERM-BF447(a-e) are five maize seed powder materials certified for their mass fraction of DP915635, (unique identifier code DP-915635-4) produced within the scope of ISO 17034 accreditation.
Genetically modified (GM) maize seeds of the DP915635 event and seeds from a non-GM maize variety were processed to GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize seed powder were prepared gravimetrically. The certified reference materials (CRMs) are available in glass vials containing at least 1 g of dried maize powder, which were sealed under an atmosphere of argon. The minimum sample size for one measurement is 200 mg.
Between-unit homogeneity, stability during transport and storage were assessed in accordance with ISO Guide 35:2017.
The certified values were obtained from the gravimetric preparations, taking into account the genetic purity of the non-GM and GM maize powders with respect to the DP915635 maize event and their water mass fractions. The certified values were confirmed by event-specific quantitative polymerase chain reaction, measured within the scope of accreditation to ISO/IEC 17025:2017.
Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertaint
KORTEKAAS Anne Marie;
SEGHERS John;
GARCIA RUIZ Silvia;
CONTRERAS LOPEZ Maria Concepcion;
CORBISIER Philippe;
TRAPMANN Stefanie;
2023-12-20
Publications Office of the European Union
JRC135484
978-92-68-08642-1 (online),
1831-9424 (online),
EUR 31712 EN,
OP KJ-NA-31-712-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC135484,
10.2760/773609 (online),
Additional supporting files
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