Assessment of the Predictivity of DIO1-SK Assay to Investigate DIO1 Inhibition in Human Liver Microsomes
The European Union Reference Laboratory for alternatives to animal testing (EU RL ECVAM) coordinated the validation of multiple thyroid in vitro assays aiming to set up an in vitro test battery. The validation consisted of two independent parts: part 1 (Reproducibility assessment) in which the reproducibility of the method is
evaluated by testing a small number of described inhibitors and part 2 (Predictivity assessment) where the predictivity of the method was investigated by testing a blinded set of substances. The deiodinases (DIO) are important regulators of thyroid hormone (TH) metabolism and potential targets for substance-induced thyroid disruption.
The EU-NETVAL laboratory previously reported the part 1 results for the Sandell–Kolthoff reaction-based type 1 deiodinase (DIO1-SK) assay and an extensive description of the method. In this study, they report the results of the part 2 testing in the DIO1-SK assay.
WEBER Andreas-Georg;
BIRK Barbara;
GIRI Varun;
HOFFMANN Sebastian;
RENKO Kostja;
COECKE Sandra;
SCHNEIDER Steffen;
FUNK-WEYER Dorothee;
LANDSIEDEL Robert;
2024-01-15
Mary Ann Liebert, Inc.
JRC135765
2332-1512 (online),
https://www.liebertpub.com/doi/full/10.1089/aivt.2022.0016,
https://doi.org/10.1089/aivt.2022.0016,
https://publications.jrc.ec.europa.eu/repository/handle/JRC135765,
10.1089/aivt.2022.0016 (online),
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