Analytical Ultracentrifugation to Assess the Quality of LNP-mRNA Therapeutics
The approval of safe and effective LNP-mRNA vaccines during the SARS-CoV2 pandemic is catalysing the development of the next generation of mRNA therapeutics. Proper characterisation methods are crucial for assessing the quality and efficacy of these complex formulations. Here we show that analytical ultracentrifugation (AUC) can measure, simultaneously and without any sample preparation step, the sedimentation coefficients of both the LNP-mRNA formulation and of the mRNA molecules. This allows measuring several quality attributes, such as particle size distribution, encapsulation efficiency and density of the formulation. The technique can also be applied to study the stability of the formulation under stress conditions and different buffers.
GUERRINI Giuditta;
MEHN Dora;
SCACCABAROZZI Diletta;
GIORIA Sabrina;
CALZOLAI Luigi;
2024-06-21
MDPI
JRC137564
1422-0067 (online),
https://www.mdpi.com/1422-0067/25/11/5718,
https://publications.jrc.ec.europa.eu/repository/handle/JRC137564,
10.3390/ijms25115718 (online),
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