Comparison of myelin formation in two 3D mixed neuronal/glial cultures (brain spheres) derived from human induced pluripotent stem cells
In the field of developmental neurotoxicity and endocrine disruption, there is a need for in vitro methods that are suitable for regulatory use and can measure specific changes in the human brain. In the search for a suitable, commercially available cell model, EURL ECVAM requested a contractor to evaluate the quantity and reproducibility of myelin formation in 3D mixed (neuronal/astrocyte and oligodendrocyte) cultures (brain spheres) derived from human induced pluripotent stem cells (hiPSCs). The results from the commercially available cell line were compared with results from an in-house model that was already established in the contractor's laboratory. The study shows that the investigated cell model produces myelin that can be reliably measured, but challenges remain for the reproducible measurement of increased or decreased myelination in the brainspheres upon chemical exposure.
PAMIES David;
WANG Shan;
2025-03-28
Publications Office of the European Union
JRC138781
978-92-68-20977-6 (online),
OP KJ-01-24-073-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC138781,
10.2760/8173533 (online),
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