A comparison of hydrodynamic diameter results from MADLS and DLS measurements for nanoparticle reference materials

Nanoparticles measurements by multi-angle dynamic light scattering

Dynamic light scattering (DLS) is an ubiquitous and highly standardised method that is used across research, development and qualification of nanoparticles. Multi-angle dynamic light scattering (MADLS) has recently been gaining popularity thanks to increased access to bench-top instrumentations, but its metrology framework is not yet as advanced as for DLS. The lack of metrology and traceability can hinder a further commercialisation of MADLS instrumentation and standardisation. In this work we utilise spherical gold, silica and polystyrene reference materials to provide a comparative dataset for the measurement of nanoparticle hydrodynamic diameters by DLS and MADLS. We also show measurements of particle number concentration by MADLS and discuss the impact of the knowledge of experimental parameters such as the particle refractive index on the measurement results. This work improves user confidence in the use of MADLS, benefits experimental design and ultimately provides some foundational data to support method verification and future standardisation.

COONES Ryan T.;  KESTENS Vikram;  MINELLI Caterina; 

2025-12-02

SPRINGER

JRC139139

1572-896X (online),   

https://doi.org/10.1007/s11051-025-06284-4,    https://publications.jrc.ec.europa.eu/repository/handle/JRC139139,   

10.1007/s11051-025-06284-4 (online),