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Certification of the mass fraction of NK603 in maize seed powder: ERM®-BF415g

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ERM®-BF415g is a genetically modified (GM) maize seed powder material certified for the mass fraction of maize event NK603 (unique identifier MON-ØØ6Ø3-6), produced within the scope of ISO 17034 accreditation and ISO 33405:2024. GM maize seeds of the event NK603 were milled, sieved and dried to obtain pure GM seed powder. The certified reference material (CRM) is available in glass vials containing at least 1 g of dried species powder, which were sealed under an argon atmosphere. Between-unit homogeneity was quantified and stability during transport and storage was assessed in accordance with ISO 33405:2024. The minimum sample size for one measurement is 200 mg. The certified value is based on the genetic purity of the base material (NK603) and it was confirmed by event-specific real-time PCR measurements performed according to ISO/IEC 17025:2017. Uncertainties of the certified value were calculated in accordance with ISO 17034:2016 and ISO 33405:2024 and include uncertainties related to possible inhomogeneity, instability and characterisation, based on the genetic purity assessment. The material is intended for the calibration or quality control or assessment of method performance of the event-specific qPCR method for NK603 maize. Before release of the CRM, the certification project was subjected to peer-review involving internal experts.
2025-03-28
Publications Office of the European Union
JRC139976
978-92-68-21964-5 (online),   
OP KJ-01-24-139-EN-N (online),   
https://publications.jrc.ec.europa.eu/repository/handle/JRC139976,   
10.2760/9415888 (online),   
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