The Adverse Outcome Pathway (AOP) framework is a pivotal tool for organizing mechanistic knowledge and linking it to adverse outcomes of regulatory significance. However, the integration of test method information, particularly New Approach Methods (NAMs), within the central repository for AOP knowledge, (the AOP-Wiki), has been suboptimal, limiting the framework's utility for regulatory decision-making. The Methods2AOP collaboration, comprised of various international stakeholders, was established to address this gap and enhance the role of test methods within the AOP framework. This paper reviews the importance of linking detailed test method information and conceptually proposes how to include it in the AOP knowledgebase. The Methods2AOP collaboration advocates for using ontologies to standardize and structure information, thereby facilitating interoperability, enabling reusability, and establishing clear connections between test methods and Key Events (KEs). The implementation of Methods2AOP recommendations is expected to increase the clarity and transparency of method descriptions, which can support regulatory acceptance and a wider adoption of NAMs. The broad community of stakeholders impacted by this work stands to benefit from the Methods2AOP recommendations through enhanced regulatory decisions, increased visibility and scientific impact, new market opportunities, and the accelerated adoption of NAMs in regulatory affairs. In summary, the Methods2AOP collaboration presents a comprehensive effort to formally standardize the integration of test methods into the AOP framework, thereby fostering a more robust, and transparent system that aligns with the goals of the scientific and regulatory communities.
KARMAUS Agnes;
BISSON William;
BRAEUNING Albert;
CHANG Xiaoqing;
CLERBAUX Laure-Alix;
FILIPOVSKA Julija;
FOSTEL Jennifer;
GROH Ksenia J.;
HENCH Virginia;
LEKKA Eftychia;
LYNN Scott G.;
MAGURANY Kelly;
MARKEY Kristan;
MASCI Anna Maria;
MORTENSEN Holly;
O'BRIEN Jason M.;
REINKE Emily;
TUCKER Nyssa;
VIRVILIS Vassilis;
VIVIANI Barbara;
VLIET Sara;
WITTWEHR Clemens;
HOGBERG Helena;
2026-06-19
F1000 RESEARCH LTD
JRC142303
2046-1402 (online),
https://doi.org/10.12688/f1000research.172881.2,
https://publications.jrc.ec.europa.eu/repository/handle/JRC142303,
10.12688/f1000research.172881.2 (online),
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