Certification of the mass fraction of cereulide toxin in cooked rice: ERM-BD300
JRC REFERENCE MATERIAL REPORT
ERM®-BD300 is a rice reference material produced within the scope of ISO 17034:2016 accreditation. It is certified for the mass fraction of the emetic toxin cereulide.
The material consists of cooked long grain white rice that was cryogenically milled, converted into a watery slurry, spiked with cereulide, freeze-dried, cryogenically milled again and mixed. The certified reference material (CRM) is available in amber glass vials containing at least 6.0 g of rice powder (equivalent to ca. 18.5 g of cooked rice).
The between-unit homogeneity was quantified and stability during transport and storage was assessed in accordance with ISO 33405:2024. The minimum sample size for one measurement is 1 g reconstituted rice.
The material was characterised by an interlaboratory comparison of laboratories of demonstrated competence and adhering to ISO/IEC 17025:2017. All laboratories applied methods in accordance with or similar to ISO 18465:2017 (quantitative cereulide determination using LC-MS/MS). Technically invalid results were removed but no outlier was eliminated unless a technical reason for the deviation was found.
Uncertainties of the certified values include uncertainties related to possible inhomogeneity, instability and characterisation.
The material is intended for the assessment of method performance and for quality control. Its certified value can be used for trueness control.
Before release of the CRM, the certification project was subjected to peer-review involving both JRC-internal experts and the co-author from Sciensano.
ZELENY Reinhard;
SEGHERS John;
2026-02-05
Publications Office of the European Union
JRC144843
978-92-68-34804-8 (online),
OP KJ-01-25-625-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC144843,
10.2760/7056138 (online),
Additional supporting files
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