Validation of Concentration, Extraction and Quantification Assay
This series of factsheets describes the validated analytical methodologies used for the detection and quantification of viral pathogens in raw wastewater, developed within the framework of the EU Super-Site Sentinel System (EU4S) Network. Each factsheet presents a harmonised, three-pillar approach encompassing viral concentration, nucleic acid extraction, and quantitative PCR-based detection, supported by internal process and extraction controls to ensure data reliability and reproducibility. The use of standardised protocols across Super-Site locations — including transportation hubs, wastewater treatment plants, hospitals, and large-event venues — enables the generation of spatially and temporally comparable surveillance data. Together, these methodologies underpin wastewater-based surveillance (WBS) as a sensitive, population-level tool for early outbreak detection, infection trend monitoring, and public health preparedness across the European Union.
PANZARELLA Giulia;
COMERO Sara;
GLOWACKA Natalia;
MONTEIRO Silvia;
SANTOS Ricardo;
PIRES Joana;
CASADO POBLADOR Tanja;
KLEIN Christophe;
GAWLIK Bernd Manfred;
2026-05-18
Publications Office of the European Union
JRC146701
978-92-68-39854-8 (online),
OP KJ-01-26-209-EN-N (online),
https://publications.jrc.ec.europa.eu/repository/handle/JRC146701,
10.2760/8755466 (online),
| Name | Country | City | Type |
|---|
This document is only visible at the Commission level.
You are not authorized to publish or distribute it outside the European Commission.
This is a public document. You can share this publication.
Datasets
| ID | Title | Public URL |
|---|
Dataset collections
| ID | Acronym | Title | Public URL |
|---|
Scripts / source codes
| Description | Public URL |
|---|
Additional supporting files
| File name | Description | File type |
|---|