The evaporative light scattering detector (ELSD) is increasingly applied in combination with high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). It was found that he has an advantage especially in the field of the analysis of triacylglycerols and other neutral lipids, showing no or only weak ultraviolet absorption. The main advantage is the possibility to apply a gradient for the better separation of these compounds. Care must be taken for the quantitative evaluation of the results. While some authors state low detection limits and linear response functions, others reported frequently non-linear response of the detector. The published data indicates, that this seems to be attributed to the design of the detector and the exact conditions of separation.
LIPP Markus;
ANKLAM Elke;
1998-03-04
JRC15943
https://publications.jrc.ec.europa.eu/repository/handle/JRC15943,
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