Electrochemical Behaviour of a Metallothionein Related Peptide in the Presence of Cadmium Using Cyclic Voltammetry.

The electrochemical study on the peptidic fragment Lys- Cys- Thr- Cys- Cys- Ala [56-61] MT I (FT) in the presence of cadmium was performed by cyclic voltammetry using a hanging mercury drop electrode in order to elucidate the diffusion-adsorption processes. The influence of scan range, number of cycles, scan rate and concentration was investigated. The variation of the initial and final potentials strongly modify the voltammetric features due to changes of concentrations of species near the electrode surface. For the 1st cycle, linear relationships were found between the peak current of the different peaks and the square root of the scan rate indicating that the electrochemical mechanism is mainly diffusion-controlled. Peak current as a function of the total concentration is also linear, at least for a given range of concentration. For concentrations of the mixture FT:Cd=1:1 higher than C~3.10 -5 M, an adsorption pre-peak was detected during the cathodic scan. The electrochemical system are reversible. The evolution of voltammograms was investigated for the sequential adition of cadmium to the peptidic fragment.

HARLYK Charlotte;  BORDIN Guy;  NIETO Palmeiro Oscar;  RODRIGUEZ-FERNANDEZ Adela; 

1998-06-09

JRC16113

https://publications.jrc.ec.europa.eu/repository/handle/JRC16113,