Validation of a Screening Method Based on PCR for the Detection of GMO in Food.

A procedure for the validation of a screening method suitable for the detection of most of the transgenic plants in food was described. This method is based on the detection of two regular sequences, the 35S promotor and the NOS terminator, by PCR, the ploymerase chain reaction. The design outlined here provides a flexible approach as it allows great flexibility on the individual techniques used for DNA extraction, purification and amplification. However, detailed documentation of the results is required that allows an in-depth evaluation and comparison of the analytical results obtained by the individual laboratories.

LIPP Markus; 

1998-06-08

JRC16490