The effects of two substituted polychlorinated biphenyls, the 3, 4, 5, 3', 4', 5' (PCB 169) and the 2, 3, 4, 2', 4', 5', (PCB 138) were examined on the expression of c-myc, c-jun, c-ras and jun-b in 3T3-L1 cells.
Northern blot analysis demonstrated that the two PCBs, which exhibit a coplanar and di-ortho substituted configuration, activated these oncogenes differently.
PCB 138 markedly induced overexpression of ras, jun and myc whereas PCB 169 led to the overexpression of jun-b. HPLC analysis of the cell samples treated in medium without serum revealed a higher intracellular concentration of the 2,3,4, 2', 4', 5', Hexaclorobiphenyl (HexaCB), whereas the 3, 4, 5, 3', 4', 5', HexaCB reached the same concentration in the sonicated samples of cells with or without serum.
These results indicated that there was a relationship between PCBs structure, bioavaliability and the capacity to stimulate oncogene expression.
GRIBALDO Laura;
SACCO Maria Grazia;
CASATI Silvia;
ZUCCHI Ileana;
DOSANJH Manjit;
CATALANI Paolo;
MARAFANTE Erminio;
1998-09-17
JRC16695
