Reference Materials (RMs) for the Analysis of the Human Factor II (prothrombin) Gene G20210A Mutation

The Scientific Committee of Molecular Biology Techniques (C-MBT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission - Joint Research Centre, Institute of Reference Materials and Measurments to develop and produce plasmidic-type reference materials (RMs) for the analysis of the human prothrombin gene G20210A mutation. Although DNA tests have a high impact on the clinical decision-making, and the number of tests performed in diagnostic laboratories is high, issues of quality and quality assurance exist, and currently only a few RMs for clinical genetic testing are available. A gene fragment chosen was produced that spans all primer annealing sites published until today. Both, the wildtype and the mutant allele of this gene fragment were cloned into a pUC18 plasmid and two plasmidic RMs were produced. In addition a mixture of both plasmids was produced mimicking the heterozygous genotype. The present study describes the performance and results of these reference materials in commutability study, when they were tested by 9 different methods in 13 expert laboratories. This series of plasmidic RMs are to the best of our knowledge the world-wide first plasmidic-type clinical genetic RMs introduced.

KLEIN Christoph;  MARKI-ZAY Janos;  CORBISIER Philippe;  GANCBERG David;  COOPER Susan;  GEMMATI Donato;  HALBMAYER Walter-Michael;  KITCHEN Steve;  MELEGH Bela;  NEUMAIER Michael;  OLDENBURG Johannes;  OPPLIGER LEIBUNDGUT Elisabeth;  REITSMA Pieter;  RIEGER Sandra;  SCHIMMEL Heinz;  SPANNAGL Michael;  TORDAI Attila;  TOSETTO Alberto;  VISVIKIS Sophie;  MANNHALTER Christine; 

ZADRO Renata; 

2005-12-20

WALTER DE GRUYTER & CO

JRC30316

https://publications.jrc.ec.europa.eu/repository/handle/JRC30316,   

10.1515/CCLM.2005.145,