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dc.contributor.authorZUCCO Flaviaen_GB
dc.contributor.authorBATTO Anne-Françoiseen_GB
dc.contributor.authorBISES Giovannaen_GB
dc.contributor.authorCHAMBAZ Jeanen_GB
dc.contributor.authorCHIUSOLO Ariannaen_GB
dc.contributor.authorCONSALVO Rosaen_GB
dc.contributor.authorCROSS Heideen_GB
dc.contributor.authorDAL NEGRO Giannien_GB
dc.contributor.authorDE ANGELIS Isabellaen_GB
dc.contributor.authorFABRE Gérarden_GB
dc.contributor.authorGUILLOU Françoisen_GB
dc.contributor.authorHOFFMANN SEBASTIANen_GB
dc.contributor.authorLAPLANCHE Loicen_GB
dc.contributor.authorMOREL Etienneen_GB
dc.contributor.authorPINÇON-RAYMOND Martineen_GB
dc.contributor.authorPRIETO PERAITA MARIA DEL PILARen_GB
dc.contributor.authorTURCO Lauraen_GB
dc.contributor.authorROUSSET Moniqueen_GB
dc.contributor.authorSAMBUY Yulaen_GB
dc.contributor.authorSCARINO Mariaen_GB
dc.contributor.authorTORREILLES Françoisen_GB
dc.contributor.authorSTAMMATI Annalauraen_GB
dc.date.accessioned2010-02-25T14:42:33Z-
dc.date.available2006-01-19en_GB
dc.date.available2010-02-25T14:42:33Z-
dc.date.issued2005en_GB
dc.date.submitted2006-01-13en_GB
dc.identifier.citationATLA-ALTERNATIVES TO LABORATORY ANIMALS vol. 33 p. 603-618en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC32018-
dc.description.abstractDifferentiated human intestinal Caco-2 cells are frequently used in toxicology and pharmacology as in vitro models for studies on intestinal barrier functions. Since several discrepancies exist among the different lines and clones of Caco-2 cells, comparison of the results obtained and optimisation of models for use for regulatory purposes are particularly difficult, especially with respect to culture conditions and morphological and biochemical parameters. An inter-laboratory study has been performed on the parental cell line and on three clonal Caco-2 cell lines, with the aim of standardising the culture conditions and identifying the best cell line with respect to parameters relevant to barrier integrity, namely, trans-epithelial electrical resistance (TEER) and mannitol passage, and of epithelial differentiation (alkaline phosphatase activity). Comparison of the cell lines maintained in traditional serum-supplemented culture medium or in defined medium, containing insulin, transferrin, selenium and lipids, showed that parameter performance was better and more reproducible with the traditional medium. The maintenance of the cell lines for 15 days in culture was found to be sufficient for the development of barrier properties, but not for full epithelial differentiation. Caco-2/TC7 cells performed better than the other three cell lines, both in terms of reproducibility and performance, exhibiting low TEER and mannitol passage, and high alkaline phosphatase activity.en_GB
dc.description.sponsorshipJRC.I.2-Validation of biomedical testing methodsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherFRAMEen_GB
dc.relation.ispartofseriesJRC32018en_GB
dc.titleAn Inter-laboratory Study to Evaluate the Effects of Medium Composition on the Differentiation and Barrier Function of Caco-2 Cell Linesen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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