Control of Cell Adhesion and Spreading by Spatial Microarranged PEO-like and pdAA Domains

Microstructured surfaces are widely used in cell culture experiments to understand the fundamentals of cell-material interactions by a spatial control of cell adhesion and spreading. Recent studies have documented that both substrate chemistry and topography are tightly correlated to cell behaviours. For this reason a wide range of techniques have been explored for obtaining in a simple and cheap way reproducible patterned substrates. This paper describes how to produce micropatterned substrates by a spatial microarrangment of chemically different domains, produced by plasma deposition. Cell-repulsive zones, obtained by plasma deposited PolyethyleneOxide-like (PEO-like) coating, were alternated with cell-adhesive tracks, namely plasma deposited Acrylic Acid (pdAA) films. Time lapse experiments demonstrated that such patterns, suitable to exert chemical and topographical constraints for cell-adhesion, can also support migration of cells inside the produced pattern.

SARDELLA E.;  GRISTINA R.;  CECCONE Giacomo;  GILLILAND Douglas;  PAPADOPOULOU Andri;  ROSSI Francois;  SENESI G.S.;  DETOMASO L.;  FAVIA P.;  D'AGOSTINO R.; 

2006-01-19

Elsevier B.V.

JRC32021

https://publications.jrc.ec.europa.eu/repository/handle/JRC32021,   

10.1016/j.surfcoat.2005.02.06,