Development of an innovative immunoassay for CP4EPSPS and Cry1AB GM proteins detection and quantification

An innovative immunoassay, called ELISA Reverse, based on a new conformation of the solid phase was developed; the solid support was expressly designed for being immersed directly in liquid samples to detect the presence of protein targets. Its application is proposed in those cases where a large number of samples have to be screened simultaneously or when the simultaneous detection of different proteins is required. As a first application a quantitative immunoassay for Cry1AB protein in GM maize was optimised. The method was tested using GMO concentrations from 0.1 to 2%. The LOD and LOQ of the method were respectively determined equal to 0.0056 and 0.0168 (expressed in % of GMO content). A protocol to assess the presence of two GM proteins simultaneously has also been established for the case of the Cry1AB and the CP4EPSPS respectively present in GM maize and soy.

ERMOLLI Monica;  PROSPERO Adelina;  BALLA Branko;  QUERCI Maddalena;  MAZZEO Alessandra;  VAN DEN EEDE Guy; 

2006-10-16

TAYLOR & FRANCIS LTD

JRC32181

https://publications.jrc.ec.europa.eu/repository/handle/JRC32181,