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dc.contributor.authorERMOLLI MONICAen_GB
dc.contributor.authorPROSPERO ADELINAen_GB
dc.contributor.authorBALLA BRANKOen_GB
dc.contributor.authorQUERCI MADDALENAen_GB
dc.contributor.authorMAZZEO Alessandraen_GB
dc.contributor.authorVAN DEN EEDE GUYen_GB
dc.date.accessioned2010-02-25T14:41:50Z-
dc.date.available2006-10-16en_GB
dc.date.available2010-02-25T14:41:50Z-
dc.date.issued2006en_GB
dc.date.submitted2006-01-20en_GB
dc.identifier.citationFOOD ADDITIVES AND CONTAMINANTS vol. 23 no. 9 p. 876-882en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC32181-
dc.description.abstractAn innovative immunoassay, called ELISA Reverse, based on a new conformation of the solid phase was developed; the solid support was expressly designed for being immersed directly in liquid samples to detect the presence of protein targets. Its application is proposed in those cases where a large number of samples have to be screened simultaneously or when the simultaneous detection of different proteins is required. As a first application a quantitative immunoassay for Cry1AB protein in GM maize was optimised. The method was tested using GMO concentrations from 0.1 to 2%. The LOD and LOQ of the method were respectively determined equal to 0.0056 and 0.0168 (expressed in % of GMO content). A protocol to assess the presence of two GM proteins simultaneously has also been established for the case of the Cry1AB and the CP4EPSPS respectively present in GM maize and soy.en_GB
dc.description.sponsorshipJRC.I.6-Biotechnology and GMOsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherTAYLOR & FRANCIS LTDen_GB
dc.relation.ispartofseriesJRC32181en_GB
dc.titleDevelopment of an innovative immunoassay for CP4EPSPS and Cry1AB GM proteins detection and quantificationen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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