Full metadata record
DC FieldValueLanguage
dc.contributor.authorERMOLLI MONICAen_GB
dc.contributor.authorPROSPERO ADELINAen_GB
dc.contributor.authorBALLA BRANKOen_GB
dc.contributor.authorQUERCI MADDALENAen_GB
dc.contributor.authorMAZZEO Alessandraen_GB
dc.contributor.authorVAN DEN EEDE GUYen_GB
dc.identifier.citationFOOD ADDITIVES AND CONTAMINANTS vol. 23 no. 9 p. 876-882en_GB
dc.description.abstractAn innovative immunoassay, called ELISA Reverse, based on a new conformation of the solid phase was developed; the solid support was expressly designed for being immersed directly in liquid samples to detect the presence of protein targets. Its application is proposed in those cases where a large number of samples have to be screened simultaneously or when the simultaneous detection of different proteins is required. As a first application a quantitative immunoassay for Cry1AB protein in GM maize was optimised. The method was tested using GMO concentrations from 0.1 to 2%. The LOD and LOQ of the method were respectively determined equal to 0.0056 and 0.0168 (expressed in % of GMO content). A protocol to assess the presence of two GM proteins simultaneously has also been established for the case of the Cry1AB and the CP4EPSPS respectively present in GM maize and soy.en_GB
dc.description.sponsorshipJRC.I.6-Biotechnology and GMOsen_GB
dc.publisherTAYLOR & FRANCIS LTDen_GB
dc.titleDevelopment of an innovative immunoassay for CP4EPSPS and Cry1AB GM proteins detection and quantificationen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

Files in This Item:
There are no files associated with this item.

Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.