Title: Fluorescence Imaging Spectroscopy Utilising Acousto-Optic Tuneable Filters
Citation: Proceedings of SPIE - Optical Sensing and Spectroscopy vol. 5826 p. 185-193
Publisher: SPIE - The International Society for Optical Engineering
Publication Year: 2005
JRC N°: JRC32513
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC32513
Type: Articles in periodicals and books
Abstract: A novel prototype instrument for mutli-spectral imaging applications is described. The device is based on an acousto-optic tuneable filter (AOTF) that can be easily attached to many standard imaging systems (e.g. endoscope or fluorescence microscope). The instrument developed offers significant advantages over typical fixed-filter based systems in terms of flexibility, performance and diagnostic potential. The selected AOTF was designed to have a large acceptance aperture suitable for imaging applications. Any filtering centre-wavelength in the visible range (450 to 700nm) can be rapidly selected by either random access or by continuous tuning thus providing a versatile performance. The prototype instrument has been demonstrated for in-vivo applications where it was attached to the eyepiece of a commercial endoscope allowing simultaneous white light and fluorescence endoscopy. Autofluorescence of endogenous protoporphyrin IX (PpIX), a biomarker of diseased tissues undergoing an inflammatory response, was mapped in vivo on a rat model. The AOTF device was also coupled to the viewing port of a commercial fluorescence microscope thus realising a powerful fluorescence imaging spectrometer capable of detecting and mapping fluorescent biomolecules in vitro.
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