An official website of the European Union How do you know?      
European Commission logo
JRC Publications Repository Menu

Results of an Inter-laboratory Validation Study of Five Commercial ELISA Test Kits for the Determination of Peanut Proteins in Biscuits and Dark Chocolate

cover
The results of an inter-laboratory study with five commercially available peanu ELISA test kits to detects and quantify peanut residues in two food matrices (biscuit and dark chocolate) at four different concentrations( O-10 mg peanut kg-1 matrix corresponding to about 0-2.5 mg peanut protein kg-1 matrix) are reported. In general the fice ELISA test kits evaluated could detect peanut protein in the two food matrices. In three cases, the study challenged the test kits beyond their intended use for quantification below the manufacturers' defined cut-off limits. Generally, all five ELISA test kits performed well in the concentration range 5-10 mg kg-1 rather than in the low concentrations range (2.0 to 2.5 mg kg-1). The variation in the found recoveries of peanut between the different test kits had a spread of 44-191% across all concentrations. The quantification characteristics between test kits differed significantly at the very low mg kg-1 level. Two test kits performed well even at concentrations below 5 mg kg-1 with reproducibilities ot 27-36% for biscuits and 45-57% for chocolate.
2006-03-31
JRC33049
Language Citation
NameCountryCityType
Datasets
IDTitlePublic URL
Dataset collections
IDAcronymTitlePublic URL
Scripts / source codes
DescriptionPublic URL
Additional supporting files
File nameDescriptionFile type 
Show metadata record  Copy citation url to clipboard  Download BibTeX
Items published in the JRC Publications Repository are protected by copyright, with all rights reserved, unless otherwise indicated. Additional information: https://ec.europa.eu/info/legal-notice_en#copyright-notice