Determination of Aflatoxin B1 in Tiger Nut-based Soft Drinks

An analytical method for the determination of aflatoxin B1 in a tiger nut-based soft drinks named ‘horchata’ is described. The method is based on an immunoaffinity clean-up, followed by HPLC separation and fluorescence detection after electrochemical post-column derivatization. The detection limit (S/N?=?3) and the quantification limit (S/N?=?10) were 0.02 and 0.06?µg?kg-1, respectively. The mean recovery at a level of 2?µg?l-1 was 88% (n?=?6) and the coefficient of variation was 9%. The method was applied to conduct a small market survey for a beverage named ‘horchata’ that is frequently consumed by parts of the population in Southern Europe. Twenty-two samples from Spanish and Belgian supermarkets were analysed. As a result, only one sample was found to contain aflatoxin B1 at the limit of quantitation of the method.

ARRANZ HERNANDEZ Isabel;  STROKA Joerg;  NEUGEBAUER L.; 

2006-04-21

TAYLOR & FRANCIS LTD

JRC33204

https://publications.jrc.ec.europa.eu/repository/handle/JRC33204,   

10.1080/02652030500415652,