Immobilization of Antibodies on Biosensing Devices by Nanoarrayed Self-Assembled Monolayers

This work presents an original and straightforward technique for antibody immobilization onto a surface, keeping the antibody in a biologically reactive configuration. Self-assembly of molecular monolayers and plasma-based colloidal lithography were combined to create chemical nanopatterns on the surface of a biosensing device. This technique was employed to create an array of 100 nm wide motifs having a hexagonal 2-D crystalline structure, characterized by COOH-terminated nanospots in a CH3-terminated matrix. The quality control of the chemical nanopattern was carried out by combining atomic force microscopy, ellipsometry, and contact angle measurements. Enzyme-linked immunosorbent assay experiments were set up showing that the COOH/CH3 nanopatterned surface constrains the immobilization of the antibodies in a biologically reactive configuration, thus significantly improving the device performances as compared to those of more conventional nanopatterned COOH-terminated or CH3-terminated surfaces.

VALSESIA Andrea;  COLPO Pascal;  MEZIANI Tarik;  VIEIRA LISBOA Patricia;  LEJEUNE Michael;  ROSSI Francois; 

2006-11-10

AMER CHEMICAL SOC

JRC33495

https://publications.jrc.ec.europa.eu/repository/handle/JRC33495,