First Application of a Micro-sphere Based Immunoassay to GMO Detection: Quantification of Cry1Ab Protein in GM Maize

An innovative covalent micro-sphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 GM maize lines. In particular a specific protocol was developed to asses the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% (weight GMO/weight). Test linearity was achieved in the range of values from 0.1 to 3% while fluorescence saturation level occurred at 25%. The limit of detection (LOD) and quantification (LOQ) were equal to 0.018% and 0.054% respectively. The present study describes the first application of quantitative high trough put immunoassays in GMO analysis.

FANTOZZI Anna;  ERMOLLI Monica;  MARINI Massimiliano;  SCOTTI Domenico;  BALLA Branko;  QUERCI Maddalena;  LANGRELL Stephen;  VAN DEN EEDE Guy; 

2007-03-01

AMER CHEMICAL SOC

JRC33527

http://pubs.acs.org/doi/abs/10.1021/jf061506p,    https://publications.jrc.ec.europa.eu/repository/handle/JRC33527,   

10.1021/jf061506p,