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dc.contributor.authorFANTOZZI Annaen_GB
dc.contributor.authorERMOLLI MONICAen_GB
dc.contributor.authorMARINI MASSIMILIANOen_GB
dc.contributor.authorSCOTTI Domenicoen_GB
dc.contributor.authorBALLA BRANKOen_GB
dc.contributor.authorQUERCI MADDALENAen_GB
dc.contributor.authorLANGRELL STEPHENen_GB
dc.contributor.authorVAN DEN EEDE GUYen_GB
dc.identifier.citationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 1071-1076en_GB
dc.description.abstractAn innovative covalent micro-sphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 GM maize lines. In particular a specific protocol was developed to asses the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% (weight GMO/weight). Test linearity was achieved in the range of values from 0.1 to 3% while fluorescence saturation level occurred at 25%. The limit of detection (LOD) and quantification (LOQ) were equal to 0.018% and 0.054% respectively. The present study describes the first application of quantitative high trough put immunoassays in GMO analysis.en_GB
dc.description.sponsorshipJRC.I.6-Biotechnology and GMOsen_GB
dc.publisherAMER CHEMICAL SOCen_GB
dc.titleFirst Application of a Micro-sphere Based Immunoassay to GMO Detection: Quantification of Cry1Ab Protein in GM Maizeen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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