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Cells derived from superficial and deep lymph nodes of transgenic mice in which CD40L expression was deregulated were grown in vitro. After 3 months of interleukin 3 or interleukin 12 stimulation, the cells remained interleukin-independent, showed the same in vitro growth characteristics, but LIL3+ cells were tumorigenic when reinoculated in vivo in nude mice, whereas interleukin-12-treated cells did not induce tumors. Our cell lines could provide a useful model to study the perturbation of the homeostasis allowing us to elucidate the role of cytokines as modulators of differentiation in the lymphoproliferative disorders.
2006-11-15
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
JRC34154
https://publications.jrc.ec.europa.eu/repository/handle/JRC34154,   
10.1016/j.yexmp.2005.08.006,   
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