A Plasma Polymerised Allylamine Based Label Free Piezoelectric Immunosensor Platform: Characterisation and Application

During this work a poly-allylamine (ALL) based label-free piezoelectric immunoprobe using the Quartz Crystal Microbalance (QCM) was developed. Two routes for antibody (Ab) immobilisation were investigated, the first involved the use of glutaraldehyde (GA) as a cross linker and the second the use of colloid gold nanoparticles (GNP) for surface amplification. The later route in combination with the use of an orienting protein was further used in developing an immunosensor for an allergenic protein, ovalbumin (OVA). Surface characterization information for all steps involved in the fabrication of the immunoprobe, is provided in this paper. A combination of techniques such as Immaging Ellipsometry, Atomic Force Microscopy (AFM), Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and X-Ray Photoelectron Spectroscopy (XPS) were used for the characterization of the intermediate and final surfaces. Active amino groups were provided through plasma deposition of allylamine polymer film (ALL) on the quartz crystal surface and further utilized for antibody (Ab) immobilisation. Results from this study show that simple, direct piezoelectric immunoprobes can be fabricated through appropriate Ab orientation, and surface amplification techniques, without the need for labeled compounds. The combination of surface analytical, optical, and mass characterisation techniques is confirming the effectiveness of these immunosensor fabrication strategies whose analytical capacities can be extended to detect target molecules with matched antibodies.

PAPADOPOULOU Andri;  BARRERO Josefa;  LEJEUNE Michael;  BRETAGNOL Frederic;  MANSO SILVAN Miguel;  VALSESIA Andrea;  COLPO Pascal;  GILLILAND Douglas;  CECCONE Giacomo;  ROSSI Francois; 

2007-01-18

MYU K K

JRC34580

https://publications.jrc.ec.europa.eu/repository/handle/JRC34580,