In this paper the suitability of two DNA calibrants, gDNA from plant leaves and pDNA, was investigated. The PCR efficiencies, the correlation coefficients of the calibration curves and the ratios between PCR efficiencies of transgenic and endogenous sequences were compared for both calibrants. There were no significant differences between pDNA and gDNA except for the PCR efficiencies of the calibration curves for the transgene of the two tested real-time PCR methods. It is shown that pDNA is an appropriate choice for the calibration of measurements of MON 810 corn with respect to the DNA copy number ratio.
CHARELS Diana;
BROEDERS Sylvia;
CORBISIER Philippe;
TRAPMANN Stefanie;
SCHIMMEL Heinz;
EMONS Hendrik;
2007-04-26
AMER CHEMICAL SOC
JRC34998
https://publications.jrc.ec.europa.eu/repository/handle/JRC34998,
| Name | Country | City | Type |
|---|
This document is only visible at the Commission level.
You are not authorized to publish or distribute it outside the European Commission.
This is a public document. You can share this publication.
Datasets
| ID | Title | Public URL |
|---|
Dataset collections
| ID | Acronym | Title | Public URL |
|---|
Scripts / source codes
| Description | Public URL |
|---|
Additional supporting files
| File name | Description | File type |
|---|