Certification of a Reference Material of Purified Genomic DNA from Campylobacter Jejuni (NCTC 11351), Certified Reference Material IRMM-448
This report describes the production and certification of a reference material of purified genomic DNA (gDNA) of Campylobacter jejuni (NCTC 11351). This CRM (IRMM-448) supports the harmonisation and validation of PCR methods by their use as taxonomic controls in PCR reactions. The homogeneity and stability (at three different temperatures for up to 4 weeks) of the batch was assessed by monitoring the amplification of the ceuE gene by PCR, by agarose gel electrophoresis of gDNA and determination of the mass of gDNA per vial. The material is stable at -20 °C. The authenticity of the gDNA was confirmed by DNA sequence analysis of the ceuE gene. Each vial contains a certified mass of 118 ng freeze-dried gDNA, with an expanded uncertainty (UCRM) of 65 ng (k = 2).
VAN IWAARDEN Pierre;
MEEUS Nele;
PHILIPP Wolfgang;
2007-01-30
JRC35105
92-79-03469-3,
EUR 22504 EN,
OP LA-NA-22504-EN-C,
Additional supporting files
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