Title: Certification of a Reference Material of Purified Genomic DNA from Campylobacter Jejuni (NCTC 11351), Certified Reference Material IRMM-448
Publication Year: 2007
JRC N°: JRC35105
ISBN: 92-79-03469-3
Other Identifiers: EUR 22504 EN
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC35105
Type: EUR - Scientific and Technical Research Reports
Abstract: This report describes the production and certification of a reference material of purified genomic DNA (gDNA) of Campylobacter jejuni (NCTC 11351). This CRM (IRMM-448) supports the harmonisation and validation of PCR methods by their use as taxonomic controls in PCR reactions. The homogeneity and stability (at three different temperatures for up to 4 weeks) of the batch was assessed by monitoring the amplification of the ceuE gene by PCR, by agarose gel electrophoresis of gDNA and determination of the mass of gDNA per vial. The material is stable at -20 °C. The authenticity of the gDNA was confirmed by DNA sequence analysis of the ceuE gene. Each vial contains a certified mass of 118 ng freeze-dried gDNA, with an expanded uncertainty (UCRM) of 65 ng (k = 2).
JRC Directorate:Health, Consumers and Reference Materials

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