Full metadata record
DC FieldValueLanguage
dc.contributor.authorVAN IWAARDEN Pierreen_GB
dc.contributor.authorMEEUS NELEen_GB
dc.contributor.authorPHILIPP Wolfgangen_GB
dc.date.accessioned2010-02-25T18:19:14Z-
dc.date.available2007-01-30en_GB
dc.date.available2010-02-25T18:19:14Z-
dc.date.issued2007en_GB
dc.date.submitted2006-10-27en_GB
dc.identifier.isbn92-79-03469-3en_GB
dc.identifier.otherEUR 22504 ENen_GB
dc.identifier.otherOPOCE LA-NA-22504-EN-Cen_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC35105-
dc.description.abstractThis report describes the production and certification of a reference material of purified genomic DNA (gDNA) of Campylobacter jejuni (NCTC 11351). This CRM (IRMM-448) supports the harmonisation and validation of PCR methods by their use as taxonomic controls in PCR reactions. The homogeneity and stability (at three different temperatures for up to 4 weeks) of the batch was assessed by monitoring the amplification of the ceuE gene by PCR, by agarose gel electrophoresis of gDNA and determination of the mass of gDNA per vial. The material is stable at -20 °C. The authenticity of the gDNA was confirmed by DNA sequence analysis of the ceuE gene. Each vial contains a certified mass of 118 ng freeze-dried gDNA, with an expanded uncertainty (UCRM) of 65 ng (k = 2).en_GB
dc.description.sponsorshipJRC.D.2-Reference materialsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.relation.ispartofseriesJRC35105en_GB
dc.titleCertification of a Reference Material of Purified Genomic DNA from Campylobacter Jejuni (NCTC 11351), Certified Reference Material IRMM-448en_GB
dc.typeEUR - Scientific and Technical Research Reportsen_GB
JRC Directorate:Health, Consumers and Reference Materials

Files in This Item:
File Description SizeFormat 
5105 - IRMM-448_report_final_complete.pdf1.47 MBAdobe PDFView/Open


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.