A Proteomics-based Approach to Detect and Identify Major Allergens in Processed Peanut by Capillary LC-Q-TOF (MS/MS)
An MS-based method, combining reverse-phase capillary liquid chromatography (capillary LC) with quadrupole-time-of-flight tandem mass spectrometry (nano-ESI Q-TOP MS/MS) was developed with the aim to identify a set of peptides that can function as markers for peanut allergens. Emphasis was given to the identification of the three major peanut allergens Ara h1, Ara h 2 and Ara h 3, since these proteins are considered to represent more than 30% of the total protein content of peanut and are directly relevant for the allergenic potential of this food.
CHASSAIGNE Hubert;
NORGAARD Jorgen;
VAN HENGEL Adrianus;
2007-05-24
AMER CHEMICAL SOC
JRC35811
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