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dc.contributor.authorADLER Sarahen_GB
dc.contributor.authorPELLIZZER CRISTIANen_GB
dc.contributor.authorPAPARELLA Martinen_GB
dc.contributor.authorHARTUNG THOMASen_GB
dc.contributor.authorBREMER SUSANNEen_GB
dc.identifier.citationTOXICOLOGY IN VITRO vol. 20 p. 265-271en_GB
dc.description.abstractDimethyl sulfoxidc (DMSO) and ethanol are common organic solvents used for dissolving lipophilic substances for in vitro testing. However, DMSO is known to induce differentiation in embryonic stem (ES) and embryonic leratocarcinoma (EC) cells. In order to clarify if solvents like DMSO and ethanol have an influence on in vitro developmental toxicity test systems, the presented study has evaluated their effects on differentiation by using different test systems. ES and EC cells were transfected with a construct containing the rnTert promoter combined with the green fluorescent protein gene (GFP). A down-regulation of mTert, a marker for un differentiated cells, results in a lower expression of GFP, which could be measured by flow cytomctry. Taking the specific characteristics of ES and EC cells into account this effect could be a hint for the interaction of DMSO with embryonic development. Additionally, the effects of the solvents ethanol and DMSO on Oct-4 expression, another marker for undifferentiated cells, were measured in wild-type ES cells. Beth selected molecular markers demonstrated an induction of differentiation after exposure to DMSO; in wild-type ES cells at a concentration of 0.125% and in transgenic EC cells at a concentration of 0.25% DMSO. All other differences from controls, including those which attained a level of statistical significance, were minor or not dosage related in degree, or were not consistent over time and are, therefore, considered to be of little lexicological importance. In addition, a cytotoxicity test demonstrated that the solvents affected the employed molecular markers in non-cytotoxic concen┬Čtrations. The ES cells were the most sensitive towards the cytotoxic effects of the solvent DMSO while the EC cells were more sensitive when treated with the solvent ethanol.en_GB
dc.description.sponsorshipJRC.I.2-Validation of biomedical testing methodsen_GB
dc.titleThe Effects of Solvents on Embryonic Stem Cell Differentiationen_GB
dc.typeArticles in periodicals and booksen_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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