Concentration and Mean Degree of Polymerization of Rubus Ellagitannins Evaluated by Optimized Acid Methanolysis

Ellagitannins are a major class of phenolics largely responsible for the astringent and antioxidant properties of raspberries and blackberries. The Rubus ellagitannins constitute a complex mixture of monomeric and oligomeric tannins. Rubus oligomeric ellagitannins contain, beside the well-known ellagic acid and gallic acid moieties, the sanguisorboyl linking ester group. When exposed to acids or bases, ester bonds are hydrolyzed and the hexahydroxydiphenic acid spontaneously cyclizes into ellagic acid. This study describes a new, rapid procedure for the acid hydrolysis of Rubus ellagitannins in methanol, which results in maximal yield and enables the quantification of all the major reaction products. Additionally, the method provides the rationale for estimating the mean degree of polymerization of Rubus ellagitannins.

VRHOVSEK Urska;  PALCHETTI Arianna;  RENIERO Fabiano;  GUILLOU Claude;  MASUERO Domenico;  MATTIVI Fulvio; 

2007-01-30

AMER CHEMICAL SOC

JRC36244

https://publications.jrc.ec.europa.eu/repository/handle/JRC36244,   

10.1021/jf060404w,