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dc.contributor.authorURANI Chiaraen_GB
dc.contributor.authorMELCHIORETTO Pasqualeen_GB
dc.contributor.authorCANEVALI Carmenen_GB
dc.contributor.authorMORAZZONI Francaen_GB
dc.contributor.authorGRIBALDO LAURAen_GB
dc.date.accessioned2010-02-25T14:41:47Z-
dc.date.available2010-01-25en_GB
dc.date.available2010-02-25T14:41:47Z-
dc.date.created2010-01-25en_GB
dc.date.issued2007en_GB
dc.date.submitted2007-09-12en_GB
dc.identifier.citationTOXICOLOGY IN VITRO vol. 21 p. 314-319en_GB
dc.identifier.issn0887-2333en_GB
dc.identifier.urihttp://publications.jrc.ec.europa.eu/repository/handle/JRC36783-
dc.description.abstractCadmium is a widely distributed industrial and environmental pollutant. Principle target organs are soft tissues such as the liver, where cadmium accumulates with a biological half-life of approximately 20–30 years causing a variety of toxic responses. In HepG2, CdCl2 exposure for short periods (from 1 to 24 h) induces diVerential expression of stress proteins, including MT and hsp70. However, less is known about the stress response during a prolonged exposure to this metal. MTT assay showed a low cytotoxicity of CdCl2 (0.1, 0.5, 1, 2, 5, 10 M), over a period of 72 h. Cadmium uptake by ICP–AES technique and the corresponding expression of stress proteins (MT, hsp70) during the same prolonged time were also analysed. Results show that Cd was continuously and increasingly accumulated, at the highest of the concentrations tested. Metallothionein expression was up-regulated with a saturation curve at 48 as well as 72 h after CdCl2 exposure. High levels of MT probably confer an acquired tolerance to the stress and protection against cell injury as demonstrated by low cytotoxicity values. On the contrary, the unchanged pattern of hsp70 expression suggests that this protective mechanism, unlike other members of the family, is less involved during CdCl2 prolonged exposure.en_GB
dc.description.sponsorshipJRC.DDG.I.4-Molecular biology and genomicsen_GB
dc.format.mediumPrinteden_GB
dc.languageENGen_GB
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_GB
dc.relation.ispartofseriesJRC36783en_GB
dc.titleMetallothionein and Hsp70 Expression in HepG2 cells after prolonged Cadmium Exposureen_GB
dc.typeArticles in periodicals and booksen_GB
dc.identifier.doi10.1016/j.tiv.2006.08.014en_GB
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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