Selective Release of DNA from the Surface of Indium-Tin Oxide Thin Electrode Films Using Thiol-Disulphide Exchange Chemistry

A new challenge in biointerfacial science is the development of dynamic surfaces with the ability to adjust and tune the chemical functionality at the interface between the biological and non-biological entities. This paper describes fabrication of indium tin oxide (ITO) electrodes and the design of a ligand that can be switched to enable selectively controlled interactions with DNA. Tailoring the surface composition of the ITO electrode to optimise its optical and electrical properties was also studied. The surface attachment chemistry investigated utilises thiol-disulphide exchange chemistry. This chemistry involved the covalent attachment of a thiol functionalised silane anchor to a hydroxyl activated ITO electrode surface. Subsequent reaction with 2-(2-pyridinyldithio)ethaneamine hydrochloride formed the disulphide bridge and provided the terminal amine group, which facilitates addition of a cross-linker. DNA was then covalently bound to the crosslinker and hybridisation with the complementary Cy3 labelled target DNA was achieved. Selective release of the attached DNA was demonstrated by both chemical and electrical reduction of the disulphide bond. The surface chemistry was then recycled and re-hybridisation of the target DNA was achieved. The ability to control specific release of biomolecules has applications for the development of novel biosensor platforms and a range of medical devices.

MOORE Eric J.;  CURTIN Maeve;  IONITA Justin;  MAGUIRE Anita R.;  CECCONE Giacomo;  GALVIN Paul; 

2008-01-31

AMER CHEMICAL SOC

JRC36908

0003-2700,   

https://publications.jrc.ec.europa.eu/repository/handle/JRC36908,   

10.1021/ac0618324,