This document provides the first general overview on PCR primers used by Member State control laboratories for the detection, identification and quantification of GMOs. The data has been collected from a survey launched in 2005.
The survey aimed at reviewing the PCR primers used by ENGL members for control purposes and at gathering analytical details on the corresponding PCR procedures. It further aimed to provide a catalogue of DNA sequences as a basis for the future development of plasmid standards.
Participants to the survey were specifically requested to provide information on the genetic targets and if relevant, the GM event for which the primers were designed, to include the sequences of primers and the purpose (qualitative/quantitative and screening/identification analysis), type (i.e. single, multiplex, competitive, double-competitive, simplex real-time or multiplex real-time) and specificity (gene-specificity, construct specificity or event-specificity) of the corresponding PCR assay. They were further requested to provide comments and references, as well as to specify the confidentiality status of data provided.
As a quality control step, the collated information was checked against the corresponding data
published in the GMOs methods database
(
BONFINI Laura;
MOENS William;
BEN Enrico;
QUERCI Maddalena;
AYGUN Birep;
CORBISIER Philippe;
MORISSET Dany;
ZEL Jana;
VAN DEN EEDE Guy;
2008-01-22
OPOCE
JRC37001
978-92-79-08006-7,
1018-5593,
EUR 23059 EN,
OP LB-NA-23059-EN-C,
https://publications.jrc.ec.europa.eu/repository/handle/JRC37001,
10.2788/5861,
