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|Title:||Simultaneous Determination of Nine Intense Sweeteners in Foodstuffs by High Performance Liquid Chromatography and Evaporative Light Scattering Detection - Development and Single-Laboratory Validation|
|Authors:||WASIK Andrzej; MCCOURT JOSEPHINE; BUCHGRABER MANUELA|
|Citation:||JOURNAL OF CHROMATOGRAPHY A vol. 1157 no. 1-2 p. 187-196|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in periodicals and books|
|Abstract:||A high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) has been developed for the simultaneous determination of multiple sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks, canned or bottled fruits and yoghurt. The procedure involves an extraction of the nine sweeteners with a buffer solution, sample clean-up using solid-phase extraction cartridges followed by an HPLC-ELSD analysis. The trueness of the method was satisfactory with recoveries ranging from 93 to 109 % for concentration levels around the maximum usable dosages for authorised sweeteners and from 100 to 112 % for unauthorised compounds at concentration levels close to the limit of quantification (LOQs). Precision measures showed mean repeatability values of <4 % (expressed as relative standard deviation) for highly concentrated samples and <5 % at concentration levels close to the LOQs. Intermediate precision was in most cases <8 %. The limits of detection (LODs) were below 15 µg g-1 and the LOQs below 30 µg g-1 in all three matrices. Only dulcin showed slightly higher values, i.e., LODs around 30 µg g-1 and LOQs around 50 µg g-1.|
|JRC Directorate:||Health, Consumers and Reference Materials|
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