Spectroscopic Imaging Using Acousto-Optic Tuneable Filters
We report on a novel hyper-spectral fluorescence imaging filter-module based on an
acousto-optic tuneable filter (AOTF). The AOTF functions as a full-field tuneable
bandpass filter which offers fast continuous or random access tuning with high
filtering efficiency. Due to the diffractive nature of the device, the unfiltered zeroorder
and the filtered first-order images are geometrically separated. The module
developed exploits this feature to simultaneously route both the transmitted whitelight
image and the filtered fluorescence image to two separate cameras. Incorporation
of prisms in the optical paths and careful design of the relay optics in the filter module
has overcome a number of aberrations inherent to imaging through AOTFs, leading to
excellent spatial resolution. A number of practical uses of this technique, both for in
vivo auto-fluorescence endoscopy [1] and in vitro fluorescence microscopy [2] were
reported. We will describe the operational principle and design of two recently
improved prototype instruments for fluorescence-based diagnostics and will
demonstrate their performance by presenting challenging hyper-spectral fluorescence
imaging applications.
[1] Mounir Bouhifd, Maurice P Whelan, Marc Aprahamian "Fluorescence imaging
spectroscopy utilising acousto-optic tuneable filters" Proc. SPIE Int. Soc. Opt. Eng.
5826, 185-193 (2005)
[2] Mounir Bouhifd, Maurice P. Whelan, Marc Aprahamian "Use of acousto-optic
tuneable filters for imaging fluorescence spectroscopy applications in vivo and in
vitro" Proc. SPIE Int. Soc. Opt. Eng. 5692, 11-20 (2005)
WHELAN Maurice;
BOUHIFD Mounir;
2007-07-26
SPIE
JRC37508
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