Title: Event-Specific Method for the Quantification of Oilseed Rape Line RT73 Using Real-Time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction of Oilseed Rape
Publisher: Publications Office of the European Union
Publication Year: 2017
JRC N°: JRC37550
ISBN: 978-92-79-70911-1
ISSN: 1831-9424
Other Identifiers: EUR 22918 EN
OP KJ-1A-22918-EN-N
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC37550
DOI: 10.2760/434068
Type: EUR - Scientific and Technical Research Reports
Abstract: The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF) (see Regulation EC 1829/2003), in collaboration with the European Network of GMO Laboratories (ENGL), has carried out an international collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the RT73 transformation event in oilseed rape DNA (unique identifier MON-øøø73-7). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance to Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and to Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (whole oilseed rape seed containing the transformation event and whole conventional oilseed rape seeds), whereas the JRC extracted the DNA and prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved fourteen laboratories from twelve European Countries. The results of the international collaborative trial met the ENGL performance requirements and the scientific understanding about satisfactory method performance. Therefore, the CRL-GMFF considers the method validated as fit for the purpose of regulatory compliance. The results of the collaborative study are made publicly available under http://gmo-crl.jrc.it/. The method will also be submitted to ISO 21570 for consideration as an international standard.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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