Direct Nanopatterning of 3D Chemically Active Structures for Biological Applications

A simple and reliable method to create active chemical functionalities at the nanometer scale with a 3D shape, based on techniques that allow fabrication over large-scale areas (electron-beam and plasma processes) without the use of organic solvents was proposed. The patterning procedure was applied to the fabrication of sub-micrometer features containing COOH functionalities, which permit the immobilization of biomolecules via covalent bonding through classical carbodiimide chemistry. The thickness of the layer was adjusted to ca. 20 nm by tuning the deposition time to maintain the conductive properties of the substrate and, thus, avoid a charging effect during electron-beam writing. Fluorescent optical microscopic results show that green fluorescence is observed with a high signal-to-background contrast only on the activated patterns, whereas no contrast in fluorescence is detected on the non-activated surfaces.

BRETAGNOL Frederic;  VALSESIA Andrea;  SASAKI Takao;  CECCONE Giacomo;  COLPO Pascal;  ROSSI Francois; 

2008-01-17

WILEY-V C H VERLAG GMBH

JRC40414

0935-9648,   

http://www3.interscience.wiley.com/cgi-bin/fulltext/114286149/PDFSTART?CRETRY=1&SRETRY=0,    https://publications.jrc.ec.europa.eu/repository/handle/JRC40414,   

10.1002/adma.200602523,