Software, named Cardio Analyser, was developed for digital movie analysis of beating frequencies, drug induced chronotropic effects and beating areas of contracting cardiomyocyte cultures. A major novelty of the software is the introduction of automated noise filtering and automated movie analysis of beating frequencies and areas of contracting cardiomyocyte cultures. The software was based on the observation that the intensity of light transmitted through a contractive tissue changes periodically in a way which correlates with the contractions. We provided proof of principle for the method by derivation of relevant data from movies of multicellular cardiomyocyte cultures derived from embryonic stem cells. Moreover, we compared the data to equivalent results obtained by extracellular electric field potential recordings and demonstrated higher sensitivity to chronotropic effects of isoprenaline and better cardiac differentiation of ES cells in the experimental set-up applied for movie analysis. Our study indicates that the movie analysis method may have potential to be optimised for screening in early drug discovery, aiming to identify potential cardiac drug candidates or to alerts for adverse effects on heart functionality or embryonic heart development.

STUMMANN Tina;  WRONSKI Mateusz;  SOBANSKI Tomasz;  KUMPFMUELLER Benjamin;  HARENG Lars;  BREMER Susanne;  WHELAN Maurice; 

2008-07-11

MARY ANN LIEBERT INC

JRC41975

1540-658X,   

http://www.liebertonline.com/doi/pdfplus/10.1089/adt.2008.129,    https://publications.jrc.ec.europa.eu/repository/handle/JRC41975,   

10.1089/adt.2008.129,