Nanostructured polydimethylsiloxane stamps have been used to pattern regular nano-arrays of proteins on polyethylene glycol surfaces by
microcontact printing. The master was fabricated by using colloidal lithography. In this technique polystyrene nanobeads were used as
nanomasks during subsequent oxygen etching and silica deposition. The elastomeric stamps, fabricated by classical replica moulding, reproduced accurately the nanosized features of the moulds. Printing of the proteins was performed by controlling the pressure, enabling the creation of an ordered arrangement of fouling domains (protein) on a non-fouling background (polyethylene glycol). 300 nm diameter poly-L-lysine spots spaced 100 nm were patterned. Such nanofabricated biofunctional surfaces offer promising capabilities for the development of highly compacted, ultra-sensitive biosensing devices.
RUIZ Ana Maria;
VALSESIA Andrea;
BRETAGNOL Frederic;
COLPO Pascal;
ROSSI Francois;
2008-02-19
IOP PUBLISHING LTD
JRC42180
0957-4484,
https://iopscience.iop.org/article/10.1088/0957-4484/18/50/505306/meta,
https://publications.jrc.ec.europa.eu/repository/handle/JRC42180,
10.1088/0957-4484/18/50/505306,
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