Title: Gene Expression in Nanotoxicology: a Search for Biomarkers of Exposure to Cobalt Particles and Ions
Citation: Nanotoxicology vol. 1 no. 3 p. 198-203
Publisher: Informa Healthcare
Publication Year: 2007
JRC N°: JRC42242
ISSN: 1743-5390
URI: http://www.informaworld.com/smpp/content~content=a782739280~db=all~order=page
DOI: 10.1080/17435390701644399
Type: Articles in periodicals and books
Abstract: Despite the wide use of nanoscale materials in several industrial applications as well as in biology and medicine, very little research has been carried out on the potential toxicity of nanoparticles. We had previously obtained 10 differentially expressed mRNAs in BALB3T3 fibroblasts exposed to different forms of cobalt, i.e., microparticles, nanoparticles, and ions. Those genes represented candidate biomarkers for indicating specific cellular effects after cobalt nanoparticle exposure. In the present paper, we have further evaluated the expression of those genes by real-time RT-PCR after exposure to different forms of cobalt. Moreover, we also tested some genes associated with cobalt toxicity, such as VEGF, HIF-1a, and Bnip3. We identified biomarkers that are sensitive to cobalt ions that we think to be the reactive form. Our data, in fact, are consistent with the possibility that Co-nano, due to their large surface area, once inside the cell dissolve and act as ions.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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